1) Magnitude of the load
2) frequency of the load
You can fatigue a component by loading it with a large load for a few cycles (low frequency) or a small load for many cycles (high frequency). Generally components in aircraft and other machinery break as a result of the latter. Vibration is the biggest killer here - i.e. very low load, but high frequency.
Another aspect to consider is the natural harmonic frequency of a component or assembly. When the source of your vibration (primarily the engine in the case of a trike) generates a frequency that is close or equal to the natural harmonic frequency of the component, it will be excited to a state where it will fail (just like the soprano's singing breaks a glass)
there is a classic case where a suspension bridge was built in the USA over a canyon where they did not take the effect of the wind into account. The bridge could easily handle the maximum loads that it was designed to carry, however on one fateful day the wind blew out of just the right angle and strength which caused the cables of the bridge to vibrate. This vibration was close enough to the harmonic frequency of the bridge system and this caused it to start vibrating in unison, gradually growing bigger and bigger until the whole bridge was twisting from side to side. the film that was taken at the time (it must be on youtube!) showed a guy walking on the centre line of the road with the sidewalks twisting up and down in a sinisoidal manner. Shortly thereafter the bridge failed completely.
So just a word of warning dont just look at the ultimate loading at a relatively low frequency - the vibration from your engine is always (hopefully!!) there when you fly probably the biggest portential problem that you may face from a fatigue perspective.
You can ensure that this is overcome by allowing sufficient flex in your structures and isolation of vibration (engine mountings etc.) look closely at how other similar trikes are built (you clearly have) - particularly the commercially available ones to get clues as to how the brackets etc are designed. be careful of using too small a radius on bracket corners since this is where cracks start.
As another side example. A riveted structure often provides better fatigue resistance than a welded structure. as an example welding has replaced the use of riviting in most steel structures (ships & civil structures) post WW2, however you will still find rivits are used in the ladderframe chassis' of trucks. the reason is that it can carry the load but allow sufficient flexability to absorb vibration and twisting loads without succumbing to fatigue failures.
Hope this helps and that I dont have it horribly wrong !

Good luck with your project - its very exciting and necessary to get to make aviation more affordable!
